Anti-DCAF13 antibody (298-597) (STJ115179)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DCAF13 (298-597) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DCAF13 |
| Gene ID | 25879 |
| Uniprot ID | DCA13_HUMAN |
| Immunogen Region | 298-597 |
| Immunogen Sequence | EEEPLHTILGKTVYTGIDHH WKEAVFATCGQQVDIWDEQR TNPICSMTWGFDSISSVKFN PIETFLLGSCASDRNIVLYD MRQATPLKKVILDMRTNTIC WNPMEAFIFTAANEDYNLYT FDMRALDTPVMVHMDHVSAV LDVDYSPTGKEFVSASFDKS IRIFPVDKSRSREVYHTKRM QHVICVKWTSDSKYIMCGSD EMNIRLWKANASEKLGVLTS REKAAKDYNQKLKEKFQHY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human DCAF13 (NP_056235.4). |
Additional Info
| Tissue Specificity | Expressed in the endometrium during decidualization. Expression is down-regulated in preeclampsia decidual tissues. |
| Function | Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Participates in the 18S rRNA processing in growing oocytes, being essential for oocyte nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) transition. Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex that plays a key role in embryo preimplantation and is required for normal meiotic cycle progression in oocytes. Acts as a maternal factor that regulates oocyte and zygotic chromatin tightness during maternal to zygotic transition. Also involved in the transformation of the endometrium into the decidua, known as decidualization, providing a solid foundation for implantation of blastocysts. Recognizes the histone methyltransferases SUV39H1 and SUV39H2 and directs them to polyubiquitination and proteasomal degradation, which facilitates the H3K9me3 removal and early zygotic gene expression, essential steps for progressive genome reprogramming and the establishment of pluripotency during preimplantation embryonic development. Supports the spindle assembly and chromosome condensation during oocyte meiotic division by targeting the polyubiquitination and degradation of PTEN, a lipid phosphatase that inhibits PI3K pathway as well as oocyte growth and maturation. Targets PMP22 for polyubiquitination and proteasomal degradation. |
| Protein Name | Ddb1- And Cul4-Associated Factor 13Wd Repeat And Sof Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-6790901Reactome: R-HSA-6791226Reactome: R-HSA-8951664 |
| Cellular Localisation | NucleusNucleolusIn The NucleolusLocalizes Predominantly In The Granular ComponentBut Also Detected In The Fibrillar Center And Dense Fibrillar Component |
| Alternative Antibody Names | Anti-Ddb1- And Cul4-Associated Factor 13 antibodyAnti-Wd Repeat And Sof Domain-Containing Protein 1 antibodyAnti-DCAF13 antibodyAnti-WDSOF1 antibodyAnti-HSPC064 antibody |
Information sourced from Uniprot.org