| Host: | Goat |
| Applications: | Pep-ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-DBF4B (193-207) is suitable for use in ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments in Human Testis lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DBF4B |
| Gene ID: | 80174 |
| Uniprot ID: | DBF4B_HUMAN |
| Immunogen Region: | 193-207 |
| Accession Number: | NP_663696.1; NP_079380.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms. |
| Immunogen Sequence: | QLSLASLCVKKQQPK |
| Post Translational Modifications | Phosphorylated. |
| Function | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| Protein Name | Protein Dbf4 Homolog BActivator Of S Phase Kinase-Like Protein 1Ask-Like Protein 1Chiffon Homolog BDbf4-Related Factor 1 |
| Cellular Localisation | NucleusPredominantly Found In Soluble Fraction But Not In The Chromatin-Bound Fraction |
| Alternative Antibody Names | Anti-Protein Dbf4 Homolog B antibodyAnti-Activator Of S Phase Kinase-Like Protein 1 antibodyAnti-Ask-Like Protein 1 antibodyAnti-Chiffon Homolog B antibodyAnti-Dbf4-Related Factor 1 antibodyAnti-DBF4B antibodyAnti-ASKL1 antibodyAnti-DRF1 antibody |
Information sourced from Uniprot.org