| Short Description : | Mouse monoclonal anti-DAAM1 (N-Term) for use in ELISA, WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB/ICC |
| Host: | Mouse |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 5D3 |
| Isotype: | IgG2a |
| Conjugation: | Unconjugated |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:500ICC 1:200 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | DAAM1 |
| Gene ID: | 23002 |
| Uniprot ID: | DAAM1_HUMAN |
| Immunogen: | Clone 5D3 was generated from a recombinant protein that included amino acid residues from the N-terminal region of human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2. |
| Immunogen Region: | N-Term |
| Immunogen Sequence: | Human |
| Specificity: | The antibody detects a 125 kDa* protein corresponding to the apparent molecular mass of DAAM1 on SDS-PAGE immunoblots of human A431, K562, and mouse C2C12 cells. |
| Tissue Specificity | Expressed in all tissues examined. |
| Function | Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Together with DAAM2, required for myocardial maturation and sarcomere assembly. During cell division, may regulate RHOA activation that signals spindle orientation and chromosomal segregation. |
| Protein Name | Disheveled-Associated Activator Of Morphogenesis 1 |
| Database Links | Reactome: R-HSA-4086400Reactome: R-HSA-5663220Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148 |
| Cellular Localisation | CytoplasmCytoskeletonCilium Basal BodyPerinuclearColocalizes With Rhoa And Kank1 Around Centrosomes |
| Alternative Antibody Names | Anti-Disheveled-Associated Activator Of Morphogenesis 1 antibodyAnti-DAAM1 antibodyAnti-KIAA0666 antibody |
Information sourced from Uniprot.org
