Western blot analysis of CK8 at dilution of 1:1000

Anti-Cytokeratin 8 antibody [SM0469] (STJA0010469)

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STJA0010469
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Covered by St John's Laboratory Guarantee

Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal antibody anti-Cytokeratin 8 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality : Monoclonal
Clone ID : SM0469
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H) 1:500-1:1000
IHC/IF (H, M, R) 1:1000-1:2000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: KRT8
Gene ID: 3856
Uniprot ID: K2C8_HUMAN
Immunogen: KLH conjugated Synthetic peptide corresponding to Human CK8
Post Translational Modifications Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Name Keratin - Type Ii Cytoskeletal 8
Cytokeratin-8
Ck-8
Keratin-8
K8
Type-Ii Keratin Kb8
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleus Matrix
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 8 antibody
Anti-Cytokeratin-8 antibody
Anti-Ck-8 antibody
Anti-Keratin-8 antibody
Anti-K8 antibody
Anti-Type-Ii Keratin Kb8 antibody
Anti-KRT8 antibody
Anti-CYK8 antibody

Information sourced from Uniprot.org

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