• Immunohistochemistry analysis of paraffin-embedded human esophageal using Cytokeratin 6 (KRT6) (KRT6) Rabbit monoclonal antibody (STJ11103164) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cytokeratin 6 (KRT6) Rabbit monoclonal antibody (STJ11103164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Cytokeratin 6 antibody (465-564) [S4MR] (STJ11103164)

SKU:
STJ11103164

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 6 (KRT6) (465-564) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT6C
Gene ID: 286887
Uniprot ID: K2C6C_HUMAN
Immunogen Region: 465-564
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 465-564 of human Cytokeratin 6 (KRT6) (P48668).
Immunogen Sequence: YRKLLEGEECRLNGEGVGQV NVSVVQSTISSGYGGASGVG SGLGLGGGSSYSYGSGLGIG GGFSSSSGRAIGGGLSSVGG GSSTIKYTTTSSSSRKSYKH
Tissue Specificity Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
Protein Name Keratin - Type Ii Cytoskeletal 6c
Cytokeratin-6c
Ck-6c
Cytokeratin-6e
Ck-6e
Keratin K6h
Keratin-6c
K6c
Type-Ii Keratin Kb12
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 6c antibody
Anti-Cytokeratin-6c antibody
Anti-Ck-6c antibody
Anti-Cytokeratin-6e antibody
Anti-Ck-6e antibody
Anti-Keratin K6h antibody
Anti-Keratin-6c antibody
Anti-K6c antibody
Anti-Type-Ii Keratin Kb12 antibody
Anti-KRT6C antibody
Anti-KRT6E antibody

Information sourced from Uniprot.org

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