| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Cytokeratin 20, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 49kDa band observed in Human Colon lysates (calculated MW of 48.5kDa according to NP_061883.1). See non-biotinylated parental product's datasheet for further QC data. Recommend |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | KRT20 |
| Gene ID: | 54474 |
| Uniprot ID: | K1C20_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_061883.1 |
| Immunogen Sequence: | ETNAPRAGRDYS. |
| Post Translational Modifications | Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. |
| Function | Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. |
| Protein Name | Keratin - Type I Cytoskeletal 20 Cytokeratin-20 Ck-20 Keratin-20 K20 Protein It |
| Database Links | Reactome: R-HSA-6805567 Reactome: R-HSA-6809371 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 20 antibody Anti-Cytokeratin-20 antibody Anti-Ck-20 antibody Anti-Keratin-20 antibody Anti-K20 antibody Anti-Protein It antibody Anti-KRT20 antibody |
Information sourced from Uniprot.org