• Biotinylated STJ72885 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Cytokeratin 20, Biotinylated antibody (Internal) {Biotin} (STJ73208)

SKU:
STJ73208-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Cytokeratin 20, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: KRT20
Gene ID: 54474
Uniprot ID: K1C20_HUMAN
Immunogen Region: Internal
Accession Number: NP_061883.1
Immunogen Sequence: ETNAPRAGRDYS.
Post Translational Modifications Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Protein Name Keratin - Type I Cytoskeletal 20
Cytokeratin-20
Ck-20
Keratin-20
K20
Protein It
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 20 antibody
Anti-Cytokeratin-20 antibody
Anti-Ck-20 antibody
Anti-Keratin-20 antibody
Anti-K20 antibody
Anti-Protein It antibody
Anti-KRT20 antibody

Information sourced from Uniprot.org

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