• Western blot analysis of lysates from Jurkat cells, using Cytochrome P450 3A4/5 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of Jurkat cells using CYP3A4/5 Polyclonal Antibody

Anti-CYP3A4/5 antibody (251-300 aa) (STJ92594)

SKU:
STJ92594

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cytochrome P450 3A4 and Cytochrome P450 3A5 (251-300 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP3A5
CYP3A4
Gene ID: 1577
1576
Uniprot ID: CP3A5_HUMAN
CP3A4_HUMAN
Immunogen Region: 251-300 aa
Specificity: CYP3A4/5 Polyclonal Antibody detects endogenous levels of CYP3A4/5 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cytochrome P450 3A4/5 at the amino acid range 251-300
Post Translational Modifications Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
Function A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position. Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone. Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones. Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis. Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA). Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond. Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA). Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance. Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol). Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole. Hydroxylates antimalarial drug quinine. Acts as a 1,4-cineole 2-exo-monooxygenase.
Protein Name Cytochrome P450 3a4
1 -4-Cineole 2-Exo-Monooxygenase
1 -8-Cineole 2-Exo-Monooxygenase
Albendazole Monooxygenase
Sulfoxide-Forming
Albendazole Sulfoxidase
Cypiiia3
Cypiiia4
Cholesterol 25-Hydroxylase
Cytochrome P450 3a3
Cytochrome P450 Hlp
Cytochrome P450 Nf-25
Cytochrome P450-Pcn1
Nifedipine Oxidase
Quinine 3-Monooxygenase
Database Links Reactome: R-HSA-211981
Reactome: R-HSA-5423646
Reactome: R-HSA-9027307
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 3a4 antibody
Anti-1 -4-Cineole 2-Exo-Monooxygenase antibody
Anti-1 -8-Cineole 2-Exo-Monooxygenase antibody
Anti-Albendazole Monooxygenase antibody
Anti-Sulfoxide-Forming antibody
Anti-Albendazole Sulfoxidase antibody
Anti-Cypiiia3 antibody
Anti-Cypiiia4 antibody
Anti-Cholesterol 25-Hydroxylase antibody
Anti-Cytochrome P450 3a3 antibody
Anti-Cytochrome P450 Hlp antibody
Anti-Cytochrome P450 Nf-25 antibody
Anti-Cytochrome P450-Pcn1 antibody
Anti-Nifedipine Oxidase antibody
Anti-Quinine 3-Monooxygenase antibody
Anti-CYP3A4 antibody
Anti-CYP3A3 antibody

Information sourced from Uniprot.org

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