Anti-CYP2B6 antibody (247-259) (STJ73449)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ73449-100
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General Information

Short DescriptionGoat polyclonal antibody anti-CYP2B6 (247-259) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 75kDa band observed in lysates of cell line HepG2 and K562 (calculated MW of 56.3kDa according to NP_000758.1). Recommended concentration: 0.1-0.3ยตg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolCYP2B6
Gene ID1555
Uniprot IDCP2B6_HUMAN
Accession NumberNP_000758.1
Immunogen Region247-259
Immunogen SequenceHSVEKHRETLDPS

Additional Info

Post Translational Modifications Phosphorylation is accompanied by a decrease in enzyme activity.
Function A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2. Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs. Acts as a 1,4-cineole 2-exo-monooxygenase. Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.
Protein Name Cytochrome P450 2b6
1 -4-Cineole 2-Exo-Monooxygenase
Cypiib6
Cytochrome P450 Iib1
Database Links Reactome: R-HSA-211935
Reactome: R-HSA-211945
Reactome: R-HSA-211981
Reactome: R-HSA-211999
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 2b6 antibody
Anti-1 -4-Cineole 2-Exo-Monooxygenase antibody
Anti-Cypiib6 antibody
Anti-Cytochrome P450 Iib1 antibody
Anti-CYP2B6 antibody

Information sourced from Uniprot.org

Citations

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