• Immunohistochemistry analysis of paraffin-embedded mouse lung using CYP2B6 antibody (STJ111092) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using CYP2B6 antibody (STJ111092) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CYP2B6 antibody (STJ111092) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CYP2B6 antibody (STJ111092) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CYP2B6 antibody (160-340) (STJ111092)

SKU:
STJ111092

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYP2B6 (160-340) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP2B6
Gene ID: 1555
Uniprot ID: CP2B6_HUMAN
Immunogen Region: 160-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_000758.1).
Immunogen Sequence: SKGALMDPTFLFQSITANII CSIVFGKRFHYQDQEFLKML NLFYQTFSLISSVFGQLFEL FSGFLKYFPGAHRQVYKNLQ EINAYIGHSVEKHRETLDPS APKDLIDTYLLHMEKEKSNA HSEFSHQNLNLNTLSLFFAG TETTSTTLRYGFLLMLKYPH VAERVYREIEQVIGPHRPPE L
Tissue Specificity Expressed in liver, lung and heart right ventricle.
Post Translational Modifications Phosphorylation is accompanied by a decrease in enzyme activity.
Function A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2. Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs. Acts as a 1,4-cineole 2-exo-monooxygenase. Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.
Protein Name Cytochrome P450 2b6
1 -4-Cineole 2-Exo-Monooxygenase
Cypiib6
Cytochrome P450 Iib1
Database Links Reactome: R-HSA-211935
Reactome: R-HSA-211945
Reactome: R-HSA-211981
Reactome: R-HSA-211999
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 2b6 antibody
Anti-1 -4-Cineole 2-Exo-Monooxygenase antibody
Anti-Cypiib6 antibody
Anti-Cytochrome P450 Iib1 antibody
Anti-CYP2B6 antibody

Information sourced from Uniprot.org

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