Anti-CYP24A1 antibody (36-448) (STJ23319)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYP24A1 (36-448) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP24A1 |
| Gene ID | 1591 |
| Uniprot ID | CP24A_HUMAN |
| Immunogen Region | 36-448 |
| Immunogen Sequence | PQPREVPVCPLTAGGETQNA AALPGPTSWPLLGSLLQILW KGGLKKQHDTLVEYHKKYGK IFRMKLGSFESVHLGSPCLL EALYRTESAYPQRLEIKPWK AYRDYRKEGYGLLILEGEDW QRVRSAFQKKLMKPGEVMKL DNKINEVLADFMGRIDELCD ERGHVEDLYSELNKWSFESI CLVLYEKRFGLLQKNAGDEA VNFIMAIKTMMSTFGRMMVT PVELHKSLNTKVWQDHTLA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 36-448 of human CYP24A1 (NP_001122387.1). |
Additional Info
| Function | A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 1 -25-Dihydroxyvitamin D(3 24-Hydroxylase - Mitochondrial24-OhaseVitamin D(3 24-HydroxylaseCytochrome P450 24a1Cytochrome P450-Cc24 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579010 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-1 -25-Dihydroxyvitamin D(3 24-Hydroxylase - Mitochondrial antibodyAnti-24-Ohase antibodyAnti-Vitamin D(3 24-Hydroxylase antibodyAnti-Cytochrome P450 24a1 antibodyAnti-Cytochrome P450-Cc24 antibodyAnti-CYP24A1 antibodyAnti-CYP24 antibody |
Information sourced from Uniprot.org