| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Cytochrome P450 1A2450 (331-380 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP1A2 |
| Gene ID: | 1544 |
| Uniprot ID: | CP1A2_HUMAN |
| Immunogen Region: | 331-380 aa |
| Specificity: | CYP1A2 Polyclonal Antibody detects endogenous levels of CYP1A2 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Cytochrome P450 1A2 at the amino acid range 331-380 |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation (Probable). |
| Protein Name | Cytochrome P450 1a2 Cypia2 Cholesterol 25-Hydroxylase Cytochrome P(3450 Cytochrome P450 4 Cytochrome P450-P3 Hydroperoxy Icosatetraenoate Dehydratase |
| Database Links | Reactome: R-HSA-156581 Reactome: R-HSA-211957 Reactome: R-HSA-2142670 Reactome: R-HSA-2142816 Reactome: R-HSA-5423646 Reactome: R-HSA-9018681 Reactome: R-HSA-9027307 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Peripheral Membrane Protein Microsome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 1a2 antibody Anti-Cypia2 antibody Anti-Cholesterol 25-Hydroxylase antibody Anti-Cytochrome P(3450 antibody Anti-Cytochrome P450 4 antibody Anti-Cytochrome P450-P3 antibody Anti-Hydroperoxy Icosatetraenoate Dehydratase antibody Anti-CYP1A2 antibody |
Information sourced from Uniprot.org