| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:100 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP11B2 |
| Gene ID: | 1585 |
| Uniprot ID: | C11B2_HUMAN |
| Immunogen Region: | 234-503 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3). |
| Immunogen Sequence: | TVQLMFMPRSLSRWISPKVW KEHFEAWDCIFQYGDNCIQK IYQELAFNRPQHYTGIVAEL LLKAELSLEAIKANSMELTA GSVDTTAFPLLMTLFELARN PDVQQILRQESLAAAASISE HPQKATTELPLLRAALKETL RLYPVGLFLERVVSSDLVLQ NYHIPAGTLVQVFLYSLGRN AALFPRPERYNPQRWLDIRG SGRNFHHVPFGFGMRQCLGR RLAEAEMLLLLHHVLKHFL |
| Tissue Specificity | Expressed sporadically in the zona glomerulosa (zG) of the adrenal cortex (conventional zonation), as well as in aldosterone-producing cell clusters (APCCs) composed of morphological zG cells in contact with the capsule (variegated zonation). |
| Function | A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone. Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b2 - Mitochondrial Aldosterone Synthase Aldos Aldosterone-Synthesizing Enzyme Cypxib2 Corticosterone 18-Monooxygenase - Cyp11b2 Cytochrome P-450aldo Cytochrome P-450c18 Steroid 11-Beta-Hydroxylase - Cyp11b2 Steroid 18-Hydroxylase |
| Database Links | Reactome: R-HSA-193993 Reactome: R-HSA-194002 Reactome: R-HSA-211976 Reactome: R-HSA-5579009 |
| Cellular Localisation | Mitochondrion Inner Membrane Peripheral Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 11b2 - Mitochondrial antibody Anti-Aldosterone Synthase antibody Anti-Aldos antibody Anti-Aldosterone-Synthesizing Enzyme antibody Anti-Cypxib2 antibody Anti-Corticosterone 18-Monooxygenase - Cyp11b2 antibody Anti-Cytochrome P-450aldo antibody Anti-Cytochrome P-450c18 antibody Anti-Steroid 11-Beta-Hydroxylase - Cyp11b2 antibody Anti-Steroid 18-Hydroxylase antibody Anti-CYP11B2 antibody |
Information sourced from Uniprot.org