• Western blot analysis of extracts of various cell lines, using CYP11B1 antibody (STJ29977) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CYP11B1 antibody (234-503) (STJ29977)

SKU:
STJ29977

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYP11B1 (234-503) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP11B1
Gene ID: 1584
Uniprot ID: C11B1_HUMAN
Immunogen Region: 234-503
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B1 (NP_000488.3).
Immunogen Sequence: TVQLMFMPRSLSRWTSPKVW KEHFEAWDCIFQYGDNCIQK IYQELAFSRPQQYTSIVAEL LLNAELSPDAIKANSMELTA GSVDTTVFPLLMTLFELARN PNVQQALRQESLAAAASISE HPQKATTELPLLRAALKETL RLYPVGLFLERVASSDLVLQ NYHIPAGTLVRVFLYSLGRN PALFPRPERYNPQRWLDIRG SGRNFYHVPFGFGMRQCLGR RLAEAEMLLLLHHVLKHLQ
Tissue Specificity Expressed in the zona fasciculata/reticularis of the adrenal cortex.
Function A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (Probable).
Protein Name Cytochrome P450 11b1 - Mitochondrial
Cyp11b1
Cypxib1
Cytochrome P-450c11
Cytochrome P450c11
Steroid 11-Beta-Hydroxylase - Cyp11b1
Database Links Reactome: R-HSA-194002
Reactome: R-HSA-211976
Reactome: R-HSA-5579017
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Cytochrome P450 11b1 - Mitochondrial antibody
Anti-Cyp11b1 antibody
Anti-Cypxib1 antibody
Anti-Cytochrome P-450c11 antibody
Anti-Cytochrome P450c11 antibody
Anti-Steroid 11-Beta-Hydroxylase - Cyp11b1 antibody
Anti-CYP11B1 antibody
Anti-S11BH antibody

Information sourced from Uniprot.org

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