| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP11B2 (234-503) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:100IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP11B2 |
| Gene ID: |
1585 |
| Uniprot ID: |
C11B2_HUMAN |
| Immunogen Region: |
234-503 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3). |
| Immunogen Sequence: |
TVQLMFMPRSLSRWISPKVW KEHFEAWDCIFQYGDNCIQK IYQELAFNRPQHYTGIVAEL LLKAELSLEAIKANSMELTA GSVDTTAFPLLMTLFELARN PDVQQILRQESLAAAASISE HPQKATTELPLLRAALKETL RLYPVGLFLERVVSSDLVLQ NYHIPAGTLVQVFLYSLGRN AALFPRPERYNPQRWLDIRG SGRNFHHVPFGFGMRQCLGR RLAEAEMLLLLHHVLKHFL |
| Tissue Specificity | Expressed sporadically in the zona glomerulosa (zG) of the adrenal cortex (conventional zonation), as well as in aldosterone-producing cell clusters (APCCs) composed of morphological zG cells in contact with the capsule (variegated zonation). |
| Function | A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone. Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b2 - MitochondrialAldosterone SynthaseAldosAldosterone-Synthesizing EnzymeCypxib2Corticosterone 18-Monooxygenase - Cyp11b2Cytochrome P-450aldoCytochrome P-450c18Steroid 11-Beta-Hydroxylase - Cyp11b2Steroid 18-Hydroxylase |
| Database Links | Reactome: R-HSA-193993Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579009 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome P450 11b2 - Mitochondrial antibodyAnti-Aldosterone Synthase antibodyAnti-Aldos antibodyAnti-Aldosterone-Synthesizing Enzyme antibodyAnti-Cypxib2 antibodyAnti-Corticosterone 18-Monooxygenase - Cyp11b2 antibodyAnti-Cytochrome P-450aldo antibodyAnti-Cytochrome P-450c18 antibodyAnti-Steroid 11-Beta-Hydroxylase - Cyp11b2 antibodyAnti-Steroid 18-Hydroxylase antibodyAnti-CYP11B2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
