| Short Description : | Rabbit monoclonal anti-Cyclin B1 for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ICC/IF |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | R08-9B7 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.3 mg/mL |
| Purification: | Affinity Purified |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | Ccnb1 |
| Gene ID: | 268697 |
| Uniprot ID: | CCNB1_MOUSE |
| Immunogen: | Recombinant protein of mouse Cyclin B1 |
| Post Translational Modifications | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Deubiquitinated by USP22 during G2/M phase. Phosphorylated by PLK1 at Ser-130 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-144 was also reported to be mediated by PLK1 but Ser-130 seems to be the primary phosphorylation site. |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Protein Name | G2/Mitotic-Specific Cyclin-B1 |
| Database Links | Reactome: R-MMU-174048Reactome: -MMU-176408Reactome: -MMU-176412Reactome: -MMU-176417Reactome: -MMU-2299718Reactome: -MMU-2500257Reactome: -MMU-2565942Reactome: -MMU-2980767Reactome: -MMU-2995383Reactome: -MMU-3301854Reactome: -MMU-4419969Reactome: -MMU-6804114Reactome: -MMU-68875Reactome: -MMU-69273Reactome: -MMU-69478Reactome: -MMU-75035Reactome: -MMU-8852276Reactome: -MMU-8878166 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonMicrotubule Organizing CenterCentrosome |
| Alternative Antibody Names | Anti-G2/Mitotic-Specific Cyclin-B1 antibodyAnti-Ccnb1 antibodyAnti-Ccn-2 antibodyAnti-Ccnb1-rs13 antibodyAnti-Cycb antibodyAnti-Cycb1 antibody |
Information sourced from Uniprot.org
