Anti-Cyclin B1 antibody [R01-7H7] (STJA0033934)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Cyclin B1
General Information
| Short Description | Rabbit monoclonal anti-Cyclin B1 for use in WB, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-7H7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CCNB1 |
| Gene ID | 891 |
| Uniprot ID | CCNB1_HUMAN |
| Immunogen | A synthetic peptide of human Cyclin B1 |
Additional Info
| Post Translational Modifications | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Deubiquitinated by USP22 during G2/M phase. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
| Protein Name | G2/Mitotic-Specific Cyclin-B1 |
| Database Links | Reactome: R-HSA-113507Reactome: R-HSA-156711Reactome: R-HSA-162658Reactome: R-HSA-174048Reactome: R-HSA-176408Reactome: R-HSA-176412Reactome: R-HSA-176417Reactome: R-HSA-2299718Reactome: R-HSA-2465910Reactome: R-HSA-2500257Reactome: R-HSA-2514853Reactome: R-HSA-2565942Reactome: R-HSA-2980767Reactome: R-HSA-2995383Reactome: R-HSA-3301854Reactome: R-HSA-4419969Reactome: R-HSA-6804114Reactome: R-HSA-68875Reactome: R-HSA-69273Reactome: R-HSA-69478Reactome: R-HSA-75035Reactome: R-HSA-8852276Reactome: R-HSA-8878166Reactome: R-HSA-9725371Reactome: R-HSA-9825892 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonMicrotubule Organizing CenterCentrosome |
| Alternative Antibody Names | Anti-G2/Mitotic-Specific Cyclin-B1 antibodyAnti-CCNB1 antibodyAnti-CCNB antibody |
Information sourced from Uniprot.org