| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CX3CL1/fractalkine (214-226) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 45kDa band observed in Human Colorectal cancer lysates (calculated MW of 42.2kDa according to NP_002987.1). Recommended concentration: 0.3-1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CX3CL1 |
| Gene ID: | 6376 |
| Uniprot ID: | X3CL1_HUMAN |
| Immunogen Region: | 214-226 |
| Accession Number: | NP_002987.1 |
| Immunogen Sequence: | KTSEAPSTQDPST |
| Post Translational Modifications | A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. Processed fractalkine: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes. The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable). |
| Protein Name | Fractalkine C-X3-C Motif Chemokine 1 Cx3c Membrane-Anchored Chemokine Neurotactin Small-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine |
| Database Links | Reactome: R-HSA-380108 Reactome: R-HSA-418594 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Processed Fractalkine: Secreted |
| Alternative Antibody Names | Anti-Fractalkine antibody Anti-C-X3-C Motif Chemokine 1 antibody Anti-Cx3c Membrane-Anchored Chemokine antibody Anti-Neurotactin antibody Anti-Small-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine antibody Anti-CX3CL1 antibody Anti-FKN antibody Anti-NTT antibody Anti-SCYD1 antibody Anti-A-152E5.2 antibody |
Information sourced from Uniprot.org