• Western blot analysis of lysate from K562 cells, using CX3CL1 Antibody.
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Western blot analysis of K562 cells using Fractalkine Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CX3CL1 antibody (231-280 aa) (STJ96719)

SKU:
STJ96719

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Fractalkine (231-280 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CX3CL1
Gene ID: 6376
Uniprot ID: X3CL1_HUMAN
Immunogen Region: 231-280 aa
Specificity: Fractalkine Polyclonal Antibody detects endogenous levels of Fractalkine protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human CX3CL1 at the amino acid range 231-280
Post Translational Modifications A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. O-glycosylated with core 1 or possibly core 8 glycans.
Function Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. Processed fractalkine: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. (Microbial infection) Mediates the cytoadherence of erythrocytes infected with parasite P.falciparum (strain 3D7) with endothelial cells by interacting with P.falciparum CBP1 and CBP2 expressed at the surface of erythrocytes. The adhesion prevents the elimination of infected erythrocytes by the spleen (Probable).
Protein Name Fractalkine
C-X3-C Motif Chemokine 1
Cx3c Membrane-Anchored Chemokine
Neurotactin
Small-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Processed Fractalkine: Secreted
Alternative Antibody Names Anti-Fractalkine antibody
Anti-C-X3-C Motif Chemokine 1 antibody
Anti-Cx3c Membrane-Anchored Chemokine antibody
Anti-Neurotactin antibody
Anti-Small-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine antibody
Anti-CX3CL1 antibody
Anti-FKN antibody
Anti-NTT antibody
Anti-SCYD1 antibody
Anti-A-152E5.2 antibody

Information sourced from Uniprot.org

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