• Western blot analysis of lysates from LOVO cells, using Cullin 2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cullin 2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of Jurkat cells using CUL-2 Polyclonal Antibody diluted at 1：2000
  • Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using CUL-2 Polyclonal Antibody diluted at 1：2000

Anti-CUL2 antibody (696-745 aa) (STJ92523)

SKU:
STJ92523

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cullin-2 (696-745 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CUL2
Gene ID: 8453
Uniprot ID: CUL2_HUMAN
Immunogen Region: 696-745 aa
Specificity: CUL-2 Polyclonal Antibody detects endogenous levels of CUL-2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cullin 2 at the amino acid range 696-745
Function Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase.
Protein Name Cullin-2
Cul-2
Database Links Reactome: R-HSA-1234176
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cullin-2 antibody
Anti-Cul-2 antibody
Anti-CUL2 antibody

Information sourced from Uniprot.org

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