• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-CUBN antibody (N-Term) (STJ92520)

SKU:
STJ92520

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cubilin (N-Term) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CUBN
Gene ID: 8029
Uniprot ID: CUBN_HUMAN
Immunogen Region: N-Term
Specificity: Cubilin Polyclonal Antibody detects endogenous levels of Cubilin protein.
Immunogen: Synthesized peptide derived from the N-terminal region of human Cubilin.
Post Translational Modifications The precursor is cleaved by a trans-Golgi proteinase furin, removing a propeptide. N-glycosylated.
Function Endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake. Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1. Acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the CBLIF-cobalamin complex. Ligand binding requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
Protein Name Cubilin
460 Kda Receptor
Intestinal Intrinsic Factor Receptor
Intrinsic Factor-Cobalamin Receptor
Intrinsic Factor-Vitamin B12 Receptor
Database Links Reactome: R-HSA-196791
Reactome: R-HSA-3359462
Reactome: R-HSA-3359463
Reactome: R-HSA-8964011
Reactome: R-HSA-9758881
Cellular Localisation Apical Cell Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Coated Pit
Endosome
Lysosome Membrane
Lacks A Transmembrane Domain And Depends On Interaction With Amn For Location At The Plasma Membrane
Colocalizes With Amn And Lrp2 In The Endocytotic Apparatus Of Epithelial Cells
Alternative Antibody Names Anti-Cubilin antibody
Anti-460 Kda Receptor antibody
Anti-Intestinal Intrinsic Factor Receptor antibody
Anti-Intrinsic Factor-Cobalamin Receptor antibody
Anti-Intrinsic Factor-Vitamin B12 Receptor antibody
Anti-CUBN antibody
Anti-IFCR antibody

Information sourced from Uniprot.org

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