• Western blot analysis of extracts of various cell lines, using CTSE antibody (STJ23281) at 1:200 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Immunohistochemistry of paraffin-embedded human tonsil using CTSE antibody (STJ23281) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung using CTSE antibody (STJ23281) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using CTSE antibody (STJ23281) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CTSE antibody (STJ23281)

SKU:
STJ23281

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-CTSE is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: CTSE
Gene ID: 1510
Uniprot ID: CATE_HUMAN
Immunogen: Recombinant protein of human CTSE
Tissue Specificity Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
Post Translational Modifications Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide. Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.
Function May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
Protein Name Cathepsin E Cleaved Into - Cathepsin E Form I - Cathepsin E Form Ii
Database Links Reactome: R-HSA-2132295
Cellular Localisation Endosome
The Proenzyme Is Localized To The Endoplasmic Reticulum And Golgi Apparatus
While The Mature Enzyme Is Localized To The Endosome
Alternative Antibody Names Anti-Cathepsin E Cleaved Into - Cathepsin E Form I - Cathepsin E Form Ii antibody
Anti-CTSE antibody

Information sourced from Uniprot.org

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