• Western blot analysis of extracts of various cell lines, using Cathepsin D antibody (STJ23280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CTSD antibody (65-412) (STJ23280)

SKU:
STJ23280

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTSD (65-412) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSD
Gene ID: 1509
Uniprot ID: CATD_HUMAN
Immunogen Region: 65-412
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 65-412 of human Cathepsin D (NP_001900.1).
Immunogen Sequence: GPIPEVLKNYMDAQYYGEIG IGTPPQCFTVVFDTGSSNLW VPSIHCKLLDIACWIHHKYN SDKSSTYVKNGTSFDIHYGS GSLSGYLSQDTVSVPCQSAS SASALGGVKVERQVFGEATK QPGITFIAAKFDGILGMAYP RISVNNVLPVFDNLMQQKLV DQNIFSFYLSRDPDAQPGGE LMLGGTDSKYYKGSLSYLNV TRKAYWQVHLDQVEVASGLT LCKEGCEAIVDTGTSLMVG
Tissue Specificity Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Post Translational Modifications N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Function Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Protein Name Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-2022377
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Reactome: R-HSA-77387
Reactome: R-HSA-9018519
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
In Aortic Samples
Detected As An Extracellular Protein Loosely Bound To The Matrix
Alternative Antibody Names Anti-Cathepsin D Cleaved Into - Cathepsin D Light Chain - Cathepsin D Heavy Chain antibody
Anti-CTSD antibody
Anti-CPSD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance