| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-CTR1/SLC31A1 (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S0MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC31A1 |
| Gene ID: |
1317 |
| Uniprot ID: |
COPT1_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CTR1/SLC31A1 (NP_001850.1). |
| Immunogen Sequence: |
MDHSHHMGMSYMDSNSTMQP SHHHPTTSASHSHGGGDSSM MMMPMTFYFGFKNVELLFSG LVINTAGEMAGAFVAVFLLA MFYEGLKIARESLLRKSQVS |
| Post Translational Modifications | O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain. Proteolytic cleavage, leading to a truncated form, is facilitated by SLC31A2 and initiated preferentially by CTSL and to a minor extend by CTSB in endolysosomal compartments. In vitro, is cleaved by CTSL/cathepsin L between residues 8 and 9 from the amino terminus. A post-CTSL/cathepsin L processing occurs to yield to the fully truncated form. Sulfenylated at Cys-189 after stimulation with VEGFA, which induces SLC31A1-KDR disulfide bond formation and their co-internalization to early endosomes, driving to a sustained VEGFR2 signaling. |
| Function | High affinity copper uptake protein 1: Uniporter that mediates the transport of copper(1+) from the extracellular space to the cytoplasm, across the plasma membrane and delivers directly copper(1+) to specific chaperone such as ATOX1, via a copper(1+)- mediated transient interaction between the C-terminal domain and a copper(1+) chaperone, thus controlling intracellular copper(1+) levels. May function in copper(1+) import from the apical membrane thus may drive intestinal copper absorption. The copper(1+) transport mechanism is sodium-independent, saturable and of high-affinity. Also mediates the uptake of silver(1+). May function in the influx of the platinum-containing chemotherapeutic agents. The platinum-containing chemotherapeutic agents uptake is saturable. In vitro, mediates the transport of cadmium(2+) into cells. Also participates in the first step of copper(2+) acquisition by cells through a direct transfer of copper(2+) from copper(2+) carriers in blood, such as ALB to the N-terminal domain of SLC31A1, leading to copper(2+) reduction and probably followed by copper(1+) stabilization. In addition, functions as a redox sensor to promote angiogenesis in endothelial cells, in a copper(1+) transport independent manner, by transmitting the VEGF-induced ROS signal through a sulfenylation at Cys-189 leadin g to a subsequent disulfide bond formation between SLC31A1 and KDR. The SLC31A1-KDR complex is then co-internalized to early endosomes, driving a sustained VEGFR2 signaling. Truncated CTR1 form: Mobilizes copper(1+) out of the endosomal compartment, making copper(1+) available for export out of the cells. |
| Protein Name | High Affinity Copper Uptake Protein 1Copper Transporter 1Hctr1Solute Carrier Family 31 Member 1 Cleaved Into - Truncated Ctr1 Form |
| Database Links | Reactome: R-HSA-425410 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEarly Endosome MembraneRecycling Endosome MembraneApical Cell MembraneLate Endosome MembraneBasolateral Cell MembraneThe Localization Is Controlled By The Intra And Extra-Cellular Copper ConcentrationUnder Conditions Of Elevated Extracellular Copper ConcentrationsIt Is Rapidly Internalized By Endocytosis From The Plasma Membrane By A Clathrin- And Dynamin-Mediated Process And Degradated In Order To Prevent Intracellular Copper Accumulation And To Reduce The Transport Of The Copper Across The MembraneThe Internalized Slc31a1 Is Then Localized In Early EndosomesAndUpon A Low Extracellular Copper ConcentrationsIt Is Transported Back To The Plasma Membrane In A Rab11a-Dependent Recycling PathwayLocalizes To The Apical Membrane In Intestinal Epithelial CellsMainly Localized On The Basolateral Side Of Renal Tubular CellsLocalizes To The Neuronal Cell Body Plasma Membranes |
| Alternative Antibody Names | Anti-High Affinity Copper Uptake Protein 1 antibodyAnti-Copper Transporter 1 antibodyAnti-Hctr1 antibodyAnti-Solute Carrier Family 31 Member 1 Cleaved Into - Truncated Ctr1 Form antibodyAnti-SLC31A1 antibodyAnti-COPT1 antibodyAnti-CTR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
