• Western blot analysis of extracts of various cell lines, using CSTB antibody (STJ23253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using CSTB rabbit polyclonal antibody (STJ23253) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using CSTB rabbit polyclonal antibody (STJ23253) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CSTB antibody (1-98) (STJ23253)

SKU:
STJ23253

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSTB (1-98) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSTB
Gene ID: 1476
Uniprot ID: CYTB_HUMAN
Immunogen Region: 1-98
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTB (NP_000091.1).
Immunogen Sequence: MMCGAPSATQPATAETQHIA DQVRSQLEEKENKKFPVFKA VSFKSQVVAGTNYFIKVHVG DEDFVHLRVFQSLPHENKPL TLSNYQTNKAKHDELTYF
Function This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
Protein Name Cystatin-B
Cpi-B
Liver Thiol Proteinase Inhibitor
Stefin-B
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Cystatin-B antibody
Anti-Cpi-B antibody
Anti-Liver Thiol Proteinase Inhibitor antibody
Anti-Stefin-B antibody
Anti-CSTB antibody
Anti-CST6 antibody
Anti-STFB antibody

Information sourced from Uniprot.org

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