• Western blot analysis of extracts of various cell lines, using CST7 antibody (STJ110463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of Mouse spleen using CST7 antibody (STJ110463) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen using CST7 antibody (STJ110463) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CST7 antibody (STJ110463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CST7 antibody (20-145) (STJ110463)

SKU:
STJ110463

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CST7 (20-145) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CST7
Gene ID: 8530
Uniprot ID: CYTF_HUMAN
Immunogen Region: 20-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human CST7 (NP_003641.3).
Immunogen Sequence: GPSPDTCSQDLNSRVKPGFP KTIKTNDPGVLQAARYSVEK FNNCTNDMFLFKESRITRAL VQIVKGLKYMLEVEIGRTTC KKNQHLRLDDCDFQTNHTLK QTLSCYSEVWVVPWLQHFEV PVLRCH
Tissue Specificity Primarily expressed in peripheral blood cells and spleen.
Function Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Protein Name Cystatin-F
Cystatin-7
Cystatin-Like Metastasis-Associated Protein
Cmap
Leukocystatin
Cellular Localisation Secreted
Cytoplasm
Alternative Antibody Names Anti-Cystatin-F antibody
Anti-Cystatin-7 antibody
Anti-Cystatin-Like Metastasis-Associated Protein antibody
Anti-Cmap antibody
Anti-Leukocystatin antibody
Anti-CST7 antibody

Information sourced from Uniprot.org

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