• Immunocytochemistry staining of C6 cells fixed by anhydrous methanol for 2 h at-20℃ and using anti-CSK mouse mAb (dilution 1:50).
  • Western blot detection of CSK in C6 and Jurkat cell lysates using CSK mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.

Anti-CSK antibody [2B4-C6-A10] (STJ99130)

SKU:
STJ99130

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ICC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Tyrosine-protein kinase CSK is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 2B4-C6-A10
Conjugation: Unconjugated
Isotype: IgG2b
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSK
Gene ID: 1445
Uniprot ID: CSK_HUMAN
Specificity: This antibody detects endogenous levels of CSK and does not cross-react with related proteins.
Immunogen: Purified recombinant human CSK protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated.
Function Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Protein Name Tyrosine-Protein Kinase Csk
C-Src Kinase
Protein-Tyrosine Kinase Cyl
Database Links Reactome: R-HSA-180292
Reactome: R-HSA-202427
Reactome: R-HSA-354192
Reactome: R-HSA-389948
Reactome: R-HSA-5674135
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9013407
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9706369
Cellular Localisation Cytoplasm
Cell Membrane
Mainly Cytoplasmic
Also Present In Lipid Rafts
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Csk antibody
Anti-C-Src Kinase antibody
Anti-Protein-Tyrosine Kinase Cyl antibody
Anti-CSK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance