• Immunofluorescence analysis of U-2 OS cells using CSF2 antibody (STJ27880) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using CSF2 antibody (STJ27880) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CSF2 antibody (STJ27880) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CSF2 antibody (STJ27880) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-CSF2 antibody (20-36) (STJ27880)

SKU:
STJ27880

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSF2
Gene ID: 1437
Uniprot ID: CSF2_HUMAN
Immunogen Region: 20-36
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CSF2 (NP_000749.2).
Immunogen Sequence: MWLQSLLLLGTVACSISAPA RSPSPSTQPWEHVNAIQEAR RLLNLSRDTAAEMNETVEVI SEMFDLQEPTCLQTRLELYK QGLRGSLTKLKGPLTMMASH
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Protein Name Granulocyte-Macrophage Colony-Stimulating Factor
Gm-Csf
Colony-Stimulating Factor
Csf
Molgramostin
Sargramostim
Database Links Reactome: R-HSA-512988
Reactome: R-HSA-5673001
Reactome: R-HSA-6783783
Reactome: R-HSA-8939246
Reactome: R-HSA-912526
Cellular Localisation Secreted
Alternative Antibody Names Anti-Granulocyte-Macrophage Colony-Stimulating Factor antibody
Anti-Gm-Csf antibody
Anti-Colony-Stimulating Factor antibody
Anti-Csf antibody
Anti-Molgramostin antibody
Anti-Sargramostim antibody
Anti-CSF2 antibody
Anti-GMCSF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance