• Immunofluorescence analysis of PC-12 cells using CPT2 Rabbit polyclonal antibody (STJ114300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CPT2 Rabbit polyclonal antibody (STJ114300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using CPT2 antibody (STJ114300) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CPT2 antibody (STJ114300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CPT2 antibody (404-658) (STJ114300)

SKU:
STJ114300

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPT2
Gene ID: 1376
Uniprot ID: CPT2_HUMAN
Immunogen Region: 404-658
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 404-658 of human CPT2 (NP_000089.1).
Immunogen Sequence: ATTDSTVTVQKLNFELTDAL KTGITAAKEKFDATMKTLTI DCVQFQRGGKEFLKKQKLSP DAVAQLAFQMAFLRQYGQTV ATYESCSTAAFKHGRTETIR PASVYTKRCSEAFVREPSRH SAGELQQMMVECSKYHGQLT KEAAMGQGFDRHLFALRHLA AAKGIILPELYLDPAYGQIN HNVLSTSTLSSPAVNLGGFA PVVSDGFGVGYAVHDNWIGC NVSSYPGRNAREFLQCVEK
Function Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites. Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters.
Protein Name Carnitine O-Palmitoyltransferase 2 - Mitochondrial
Carnitine Palmitoyltransferase Ii
Cpt Ii
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-200425
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Carnitine O-Palmitoyltransferase 2 - Mitochondrial antibody
Anti-Carnitine Palmitoyltransferase Ii antibody
Anti-Cpt Ii antibody
Anti-CPT2 antibody
Anti-CPT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance