• Immunohistochemistry analysis of paraffin-embedded mouse brain using CPA6 antibody (STJ115603) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using CPA6 antibody (STJ115603) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using CPA6 antibody (STJ115603) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CPA6 antibody (STJ115603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CPA6 antibody (130-437) (STJ115603)

SKU:
STJ115603

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPA6 (130-437) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPA6
Gene ID: 57094
Uniprot ID: CBPA6_HUMAN
Immunogen Region: 130-437
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-437 of human CPA6 (NP_065094.3).
Immunogen Sequence: SLSGYNYEVYHSLEEIQNWM HHLNKTHSGLIHMFSIGRSY EGRSLFILKLGRRSRLKRAV WIDCGIHAREWIGPAFCQWF VKEALLTYKSDPAMRKMLNH LYFYIMPVFNVDGYHFSWTN DRFWRKTRSRNSRFRCRGVD ANRNWKVKWCDEGASMHPCD DTYCGPFPESEPEVKAVANF LRKHRKHIRAYLSFHAYAQM LLYPYSYKYATIPNFRCVES AAYKAVNALQSVYGVRYRY
Tissue Specificity Expressed in the hippocampus, nucleus raphe, and cortex.
Function May be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. May convert inactive angiotensin I into the biologically active angiotensin II. Releases a C-terminal amino acid, with preference for large hydrophobic C-terminal amino acids and shows only very weak activity toward small amino acids and histidine.
Protein Name Carboxypeptidase A6
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Carboxypeptidase A6 antibody
Anti-CPA6 antibody
Anti-CPAH antibody

Information sourced from Uniprot.org

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