Immunohistochemistry analysis of paraffin-embedded rat heart using COX7A1 Rabbit polyclonal antibody (STJ116921) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-COX7A1 antibody (1-79) (STJ116921)

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STJ116921
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Short Description :Rabbit polyclonal COX7A1 (1-79) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IHC-P/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:100-1:500
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:COX7A1
Gene ID:1346
Uniprot ID:CX7A1_HUMAN
Immunogen Region:1-79
Immunogen Sequence:MQALRVSQALIRSFSSTARN RFQNRVREKQKLFQEDNDIP LYLKGGIVDNILYRVTMTLC LGGTVYSLYSLGWASFPRN
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human COX7A1 (NP_001855.1).
Function Component of the mitochondrial respiratory complex IV (CIV, also named cytochrome c oxidase complex), the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The CIV complex is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Acts as an assembly factor that specifically drives the homodimerization of CIV complexes, mediating the formation of mitochondrial respiratory supercomplexes (respirasomes) containing two CIV: supercomplxes with two molecules of CIV show improved activity. Despite being highly expressed in brown adipose tissue, not required for thermogenesis.
Protein Name Cytochrome C Oxidase Subunit 7a1 - Mitochondrial
Cytochrome C Oxidase Subunit Viia-Heart
Cytochrome C Oxidase Subunit Viia-H
Cytochrome C Oxidase Subunit Viia-Muscle
Cytochrome C Oxidase Subunit Viia-M
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Reactome: R-HSA-9844594
Reactome: R-HSA-9864848
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 7a1 - Mitochondrial antibody
Anti-Cytochrome C Oxidase Subunit Viia-Heart antibody
Anti-Cytochrome C Oxidase Subunit Viia-H antibody
Anti-Cytochrome C Oxidase Subunit Viia-Muscle antibody
Anti-Cytochrome C Oxidase Subunit Viia-M antibody
Anti-COX7A1 antibody
Anti-COX7AH antibody

Information sourced from Uniprot.org

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