Anti-COX4I2 antibody (C-Term) (STJ98479)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenPurified recombinant human COX4I2 (C-terminus) protein fragments expressed in E.coli.
STJ98479
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General Information

Short DescriptionMouse monoclonal anti-Cytochrome c oxidase subunit 4 isoform 2, mitochondrial (C-Term) for use in WB, FC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/FC/IF
HostMouse
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
ConjugationUnconjugated
Concentration1 mg/mL
PurificationAffinity purification
Dilution RangeWB 1:1000-1:2000
FC 1:100-1:200
IF 1:100-1:500
FormulationLiquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCOX4I2
Gene ID84701
Uniprot IDCOX42_HUMAN
ImmunogenPurified recombinant human COX4I2 (C-terminus) protein fragments expressed in E.coli.
Immunogen RegionC-Term
SpecificityCOX4I2 Monoclonal Antibody detects endogenous levels of COX4I2 protein.

Additional Info

Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 4 Isoform 2 - Mitochondrial
Cytochrome C Oxidase Subunit Iv Isoform 2
Cox Iv-2
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Reactome: R-HSA-9864848
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 4 Isoform 2 - Mitochondrial antibody
Anti-Cytochrome C Oxidase Subunit Iv Isoform 2 antibody
Anti-Cox Iv-2 antibody
Anti-COX4I2 antibody
Anti-COX4L2 antibody

Information sourced from Uniprot.org

Citations

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