| Host: | Mouse |
| Applications: | WB/IF/FC/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Cytochrome c oxidase subunit 4 isoform 1, mitochondrial is suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 6B3 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 IF 1:200-1:1000 FC 1:200-1:400 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | COX4I1 |
| Gene ID: | 1327 |
| Uniprot ID: | COX41_HUMAN |
| Specificity: | COX4 Monoclonal Antibody detects endogenous levels of COX4 protein. |
| Immunogen: | Purified recombinant fragment of human COX4 expressed in E. Coli. |
| Function | Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. |
| Protein Name | Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial Cytochrome C Oxidase Polypeptide Iv Cytochrome C Oxidase Subunit Iv Isoform 1 Cox Iv-1 |
| Database Links | Reactome: R-HSA-5628897 Reactome: R-HSA-611105 Reactome: R-HSA-9707564 Reactome: R-HSA-9837999 Reactome: R-HSA-9864848 |
| Cellular Localisation | Mitochondrion Inner Membrane Single-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial antibody Anti-Cytochrome C Oxidase Polypeptide Iv antibody Anti-Cytochrome C Oxidase Subunit Iv Isoform 1 antibody Anti-Cox Iv-1 antibody Anti-COX4I1 antibody Anti-COX4 antibody |
Information sourced from Uniprot.org