Anti-COPB1 antibody (460-740) (STJ112510)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal COPB1 (460-740) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COPB1 |
| Gene ID | 1315 |
| Uniprot ID | COPB_HUMAN |
| Immunogen Region | 460-740 |
| Immunogen Sequence | WILGEYCSTKEDIQSVMTEI RRSLGEIPIVESEIKKEAGE LKPEEEITVGPVQKLVTEMG TYATQSALSSSRPTKKEEDR PPLRGFLLDGDFFVAASLAT TLTKIALRYVALVQEKKKQN SFVAEAMLLMATILHLGKSS LPKKPITDDDVDRISLCLKV LSECSPLMNDIFNKECRQSL SHMLSAKLEEEKLSQKKESE KRNVTVQPDDPISFMQLTAK NEMNCKEDQFQLSLLAAMG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 460-740 of human COPB1 (NP_057535.1). |
Additional Info
| Post Translational Modifications | Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8. |
| Function | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins.the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments. |
| Protein Name | Coatomer Subunit BetaBeta-Coat ProteinBeta-Cop |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-6807878Reactome: R-HSA-6811434 |
| Cellular Localisation | CytoplasmGolgi Apparatus MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic VesicleCopi-Coated Vesicle MembraneCell MembraneEndoplasmic Reticulum-Golgi Intermediate CompartmentThe Coatomer Is Cytoplasmic Or Polymerized On The Cytoplasmic Side Of The GolgiAs Well As On The Vesicles/Buds Originating From ItProteolytic Cleavage By Capn8 Triggers Translocation From Golgi To CytoplasmFound In Perinuclear Vesicular-Tubular Clusters (Vtcs) And In The Golgi Region Where Associated With VesiclesBuds And Rims Of The Golgi StackOccasionally Present At The Trans-Side Of GolgiBut Mainly Present At The Cis-Golgi Side In Transitional Areas (Ta)On So-Called Peripheral Elements (Pe) Consisting Of Tubules And Vesicles Located Between The Cup-Shaped Transitional Elements (Te) Of The Rough Endoplasmic Reticulum (Rer) And The Cis-Most Golgi CisternaePresent In CytoplasmNot Associated With Visible Coats Or MembranesWith A Minor Fraction Present On Small Clusters Of Tubules And VesiclesSome Association With High-Density And Low-Density Microsomes And Mitochondria/Nuclei FractionVery Little Found In Plasma Membrane Fraction |
| Alternative Antibody Names | Anti-Coatomer Subunit Beta antibodyAnti-Beta-Coat Protein antibodyAnti-Beta-Cop antibodyAnti-COPB1 antibodyAnti-COPB antibodyAnti-MSTP026 antibody |
Information sourced from Uniprot.org