| Host: | Rabbit |
| Applications: | IHC-F |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Contactin-6 (KLHKMSSLSSVGVQILKPSTQF) for use in IHC-F in Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | Mixed |
| Purification: | Whole serum |
| Dilution Range: | IHC 1:1000-1:5000 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at minus 20°C for increased stability, and at 2-8°C with an appropriate antiba |
| Gene Symbol: | Cntn6 |
| Gene ID: | 27256 |
| Uniprot ID: | CNTN6_RAT |
| Immunogen Region: | KLHKMSSLSSVGVQILKPSTQF |
| Specificity: | This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulation of neurons and in the trigeminal nerve root, where staining is restricted to the oligodendrocytes. Confirmed to react with rat Contactin-6. |
| Immunogen: | A synthetic peptide (KLHKMSSLSSVGVQILKPSTQF) as part of rat contactin-6 conjugated to KLH has been used as the immunogen. |
| Immunogen Sequence: | Rat |
| Tissue Specificity | Specifically expressed in neuronal cells. In brain, it is expressed in spinal cord, cerebrum and cerebellum. At 17 dpc, it is expressed in hippocampus, cerebellum, and the brain stem. Strongly expressed after birth with a maximum level between P1 and P21, which corresponds to the time frame of oligodendrogliogenesis. |
| Function | Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. May be involved in motor coordination. |
| Protein Name | Contactin-6Neural Recognition Molecule Nb-3 |
| Database Links | |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-Contactin-6 antibodyAnti-Neural Recognition Molecule Nb-3 antibodyAnti-Cntn6 antibody |
Information sourced from Uniprot.org