![Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186146/407849/STJ11106950_1__05562.1744894339.jpg?c=1 "Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.")
![Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at1:700 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186146/407851/STJ11106950_1__87217.1744894341.jpg?c=1 "Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at1:700 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and COMT knockdown (KD) 293T cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186146/407850/STJ11106950_2__29263.1744894340.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and COMT knockdown (KD) 293T cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and COMT knockdown (KD) 293T cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at1:700 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection:ECL Basic Kit <br>Exposuretime: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186146/407852/STJ11106950_2__30259.1744894342.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and COMT knockdown (KD) 293T cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at1:700 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection:ECL Basic Kit <br>Exposuretime: 10s.")
![Immunofluorescence analysis of HepG2 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/186146/407853/STJ11106950_3__85794.1744894342.jpg?c=1 "Immunofluorescence analysis of HepG2 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/186146/407849/STJ11106950_1__05562.1744894339.jpg?c=1 "Western blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (STJ11106950) at 1:700 dilution. <br/>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) ( STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | COMT |
| Gene ID: | 1312 |
| Uniprot ID: | COMT_HUMAN |
| Immunogen Region: | 51-271 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 51-271 of human COMT. (NP_000745.1). |
| Immunogen Sequence: | MGDTKEQRILNHVLQHAEPG NAQSVLEAIDTYCEQKEWAM NVGDKKGKIVDAVIQEHQPS VLLELGAYCGYSAVRMARLL SPGARLITIEINPDCAAITQ RMVDFAGVKDKVTLVVGASQ DIIPQLKKKYDVDTLDMVFL DHWKDRYLPDTLLLEECGLL RKGTVLLADNVICPGAPDFL AHVRGSSCFECTHYQSFLEY REVVDGLEKAIYKGPGSEAG P |
| Tissue Specificity | Brain, liver, placenta, lymphocytes and erythrocytes. |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. |
| Protein Name | Catechol O-Methyltransferase |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-379397Reactome: R-HSA-379398Reactome: R-HSA-9679191 |
| Cellular Localisation | Isoform Soluble: CytoplasmIsoform Membrane-Bound: Cell MembraneSingle-Pass Type Ii Membrane ProteinExtracellular Side |
| Alternative Antibody Names | Anti-Catechol O-Methyltransferase antibodyAnti-COMT antibody |
Information sourced from Uniprot.org
