Anti-Collagen III alpha 1/COL3A1 antibody (150-400) [S5MR] (STJ11102715)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Collagen III alpha 1/COL3A1 (150-400) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IF/ICC:1:100-1:800IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COL3A1 |
| Gene ID | 1281 |
| Uniprot ID | CO3A1_HUMAN |
| Immunogen Region | 150-400 |
| Immunogen Sequence | NYSPQYDSYDVKSGVAVGGL AGYPGPAGPPGPPGPPGTSG HPGSPGSPGYQGPPGEPGQA GPSGPPGPPGAIGPSGPAGK DGESGRPGRPGERGLPGPPG IKGPAGIPGFPGMKGHRGFD GRNGEKGETGAPGLKGENGL PGENGAPGPMGPRGAPGERG RPGLPGAAGARGNDGARGSD GQPGPPGPPGTAGFPGSPGA KGEVGPAGSPGSNGAPGQRG EPGPQGHAGAQGPPGPPGI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 150-400 of human Collagen III alpha 1/COL3A1 (P02461). |
Additional Info
| Post Translational Modifications | Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. |
| Function | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. |
| Protein Name | Collagen Alpha-1(Iii Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-186797Reactome: R-HSA-198933Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3000480Reactome: R-HSA-419037Reactome: R-HSA-8874081Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names | Anti-Collagen Alpha-1(Iii Chain antibodyAnti-COL3A1 antibody |
Information sourced from Uniprot.org