| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S5MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IF/ICC:1:100-1:800 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | COL3A1 |
| Gene ID: | 1281 |
| Uniprot ID: | CO3A1_HUMAN |
| Immunogen Region: | 150-400 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-400 of human Collagen III alpha 1/COL3A1 (P02461). |
| Immunogen Sequence: | NYSPQYDSYDVKSGVAVGGL AGYPGPAGPPGPPGPPGTSG HPGSPGSPGYQGPPGEPGQA GPSGPPGPPGAIGPSGPAGK DGESGRPGRPGERGLPGPPG IKGPAGIPGFPGMKGHRGFD GRNGEKGETGAPGLKGENGL PGENGAPGPMGPRGAPGERG RPGLPGAAGARGNDGARGSD GQPGPPGPPGTAGFPGSPGA KGEVGPAGSPGSNGAPGQRG EPGPQGHAGAQGPPGPPGI |
| Post Translational Modifications | Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. |
| Function | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12. |
| Protein Name | Collagen Alpha-1(Iii Chain |
| Database Links | Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-186797 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-216083 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-3000480 Reactome: R-HSA-419037 Reactome: R-HSA-8874081 Reactome: R-HSA-8948216 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
| Alternative Antibody Names | Anti-Collagen Alpha-1(Iii Chain antibody Anti-COL3A1 antibody |
Information sourced from Uniprot.org