Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715) at a dilution of 1:1000.

Anti-Collagen III alpha 1/COL3A1 antibody (150-400) [S5MR] (STJ11102715)

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STJ11102715
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Covered by St John's Laboratory Guarantee

Applications:WB/IF/ICC/IP/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Monoclonal
Clone ID :S5MR
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:1000-1:6000
IF/ICC:1:100-1:800
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen
Formulation:PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:COL3A1
Gene ID:1281
Uniprot ID:CO3A1_HUMAN
Immunogen Region:150-400
Immunogen Sequence:NYSPQYDSYDVKSGVAVGGL AGYPGPAGPPGPPGPPGTSG HPGSPGSPGYQGPPGEPGQA GPSGPPGPPGAIGPSGPAGK DGESGRPGRPGERGLPGPPG IKGPAGIPGFPGMKGHRGFD GRNGEKGETGAPGLKGENGL PGENGAPGPMGPRGAPGERG RPGLPGAAGARGNDGARGSD GQPGPPGPPGTAGFPGSPGA KGEVGPAGSPGSNGAPGQRG EPGPQGHAGAQGPPGPPGI
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 150-400 of human Collagen III alpha 1/COL3A1 (P02461).
Post Translational Modifications Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Protein Name Collagen Alpha-1(Iii Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-419037
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Iii Chain antibody
Anti-COL3A1 antibody

Information sourced from Uniprot.org

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