• Western blot analysis of mouse ovary, using COLGALT1 antibody (STJ118998) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of HeLa cells using COLGALT1 rabbit polyclonal antibody (STJ118998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using COLGALT1 rabbit polyclonal antibody (STJ118998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using COLGALT1 rabbit polyclonal antibody (STJ118998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using COLGALT1 rabbit polyclonal antibody (STJ118998) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-COLGALT1 antibody (30-230) (STJ118998)

SKU:
STJ118998

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COLGALT1 (30-230) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COLGALT1
Gene ID: 79709
Uniprot ID: GT251_HUMAN
Immunogen Region: 30-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human COLGALT1 (NP_078932.2).
Immunogen Sequence: APPGADAYFPEERWSPESPL QAPRVLIALLARNAAHALPT TLGALERLRHPRERTALWVA TDHNMDNTSTVLREWLVAVK SLYHSVEWRPAEEPRSYPDE EGPKHWSDSRYEHVMKLRQA ALKSARDMWADYILFVDADN LILNPDTLSLLIAENKTVVA PMLDSRAAYSNFWCGMTSQG YYKRTPAYIPIRKRDRRGCF A
Tissue Specificity Ubiquitous with higher levels in placenta, heart, lung and spleen.
Post Translational Modifications N-glycosylated.
Function Beta-galactosyltransferase that transfers beta-galactose to hydroxylysine residues of type I collagen. By acting on collagen glycosylation, facilitates the formation of collagen triple helix. Also involved in the biosynthesis of collagen type IV.
Protein Name Procollagen Galactosyltransferase 1
Collagen Beta(1-Ogalactosyltransferase 1
Colgalt 1
Glycosyltransferase 25 Family Member 1
Hydroxylysine Galactosyltransferase 1
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Colocalized With Plod3 And Mannose Binding Lectin/Mbl2
Alternative Antibody Names Anti-Procollagen Galactosyltransferase 1 antibody
Anti-Collagen Beta(1-Ogalactosyltransferase 1 antibody
Anti-Colgalt 1 antibody
Anti-Glycosyltransferase 25 Family Member 1 antibody
Anti-Hydroxylysine Galactosyltransferase 1 antibody
Anti-COLGALT1 antibody
Anti-GLT25D1 antibody
Anti-PSEC0241 antibody

Information sourced from Uniprot.org

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