• Western blot analysis of extracts of various cell lines, using COL6A2 antibody (STJ23195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-COL6A2 antibody (720-1019) (STJ23195)

SKU:
STJ23195

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL6A2 (720-1019) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL6A2
Gene ID: 1292
Uniprot ID: CO6A2_HUMAN
Immunogen Region: 720-1019
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 720-1019 of human COL6A2 (NP_001840.3).
Immunogen Sequence: RQKTRVFAVVITDGRHDPRD DDLNLRALCDRDVTVTAIGI GDMFHEKHESENLYSIACDK PQQVRNMTLFSDLVAEKFID DMEDVLCPDPQIVCPDLPCQ TELSVAQCTQRPVDIVFLLD GSERLGEQNFHKARRFVEQV ARRLTLARRDDDPLNARVAL LQFGGPGEQQVAFPLSHNLT AIHEALETTQYLNSFSHVGA GVVHAINAIVRSPRGGARRH AELSFVFLTDGVTGNDSLH
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Collagen VI acts as a cell-binding protein.
Protein Name Collagen Alpha-2(Vi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Membrane
Peripheral Membrane Protein
Recruited On Membranes By Cspg4
Alternative Antibody Names Anti-Collagen Alpha-2(Vi Chain antibody
Anti-COL6A2 antibody

Information sourced from Uniprot.org

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