• Western blot analysis of extracts of various cell lines, using Collagen VI/COL6A1 rabbit monoclonal antibody (STJ11102536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Collagen VI/COL6A1 Rabbit monoclonal antibody (STJ11102536) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using Collagen VI/COL6A1 rabbit monoclonal antibody (STJ11102536) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Collagen VI/COL6A1 Rabbit monoclonal antibody (STJ11102536) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using Collagen VI/COL6A1 rabbit monoclonal antibody (STJ11102536) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Collagen VI/COL6A1 Rabbit monoclonal antibody (STJ11102536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-COL6A1 antibody (37-235) [S6MR] (STJ11102536)

SKU:
STJ11102536

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Collagen VI (37-235) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL6A1
Gene ID: 1291
Uniprot ID: CO6A1_HUMAN
Immunogen Region: 37-235
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-235 of human Collagen VI/COL6A1 (P12109).
Immunogen Sequence: DLFFVLDTSESVALRLKPYG ALVDKVKSFTKRFIDNLRDR YYRCDRNLVWNAGALHYSDE VEIIQGLTRMPGGRDALKSS VDAVKYFGKGTYTDCAIKKG LEQLLVGGSHLKENKYLIVV TDGHPLEGYKEPCGGLEDAV NEAKHLGVKVFSVAITPDHL EPRLSIIATDHTYRRNFTAA DWGQSRDAEEAISQTIDTI
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Collagen VI acts as a cell-binding protein.
Protein Name Collagen Alpha-1(Vi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Vi Chain antibody
Anti-COL6A1 antibody

Information sourced from Uniprot.org

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