STJ72006 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-COL4A6 antibody (Internal) (STJ72006)

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SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72006-100
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General Information

Short DescriptionGoat polyclonal antibody anti-COL4A6 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the endoplasmic reticulum of HeLa and in the endoplasmic reticulum and membranes of U2OS cells. Recommended concentration: 10ยตg/ml.

FC:Flow c

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolCOL4A6
Gene ID1288
Uniprot IDCO4A6_HUMAN
Accession NumberNP_001838.2; NP_378667.1; NP_001274687.1; NP_001274688.1; NP_001274689.1
Immunogen RegionInternal
Immunogen SequencePSPEFETETLHNKES
SpecificityThis antibody is expected to recognize reported isoforms NP_001838.2 (iso A) , ; NP_378667.1 (iso B) , NP_001274687.1 (iso 3) , NP_001274688.1 (iso 4) and NP_001274689.1 (iso 5).

Additional Info

Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Protein Name Collagen Alpha-6(Iv Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000157
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative Antibody Names Anti-Collagen Alpha-6(Iv Chain antibody
Anti-COL4A6 antibody

Information sourced from Uniprot.org

Citations

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