Host: | Rabbit |
Applications: | IHC/IF/WB/ELISA |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-Collagen alpha-5IV chain (21-70 aa) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | COL4A5 |
Gene ID: | 1287 |
Uniprot ID: | CO4A5_HUMAN |
Immunogen Region: | 21-70 aa |
Specificity: | COL4A5 Polyclonal Antibody detects endogenous levels of COL4A5 protein. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human Collagen IV alpha5 at the amino acid range 21-70 |
Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. |
Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
Protein Name | Collagen Alpha-5(Iv Chain |
Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-186797Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-3000157Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-419037Reactome: R-HSA-8948216Reactome: R-HSA-9010553 |
Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement Membrane |
Alternative Antibody Names | Anti-Collagen Alpha-5(Iv Chain antibodyAnti-COL4A5 antibody |
Information sourced from Uniprot.org