Anti-COL4A2 antibody (1486-1712) (STJ29971)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal COL4A2 (1486-1712) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COL4A2 |
| Gene ID | 1284 |
| Uniprot ID | CO4A2_HUMAN |
| Immunogen Region | 1486-1712 |
| Immunogen Sequence | VSIGYLLVKHSQTDQEPMCP VGMNKLWSGYSLLYFEGQEK AHNQDLGLAGSCLARFSTMP FLYCNPGDVCYYASRNDKSY WLSTTAPLPMMPVAEDEIKP YISRCSVCEAPAIAIAVHSQ DVSIPHCPAGWRSLWIGYSF LMHTAAGDEGGGQSLVSPGS CLEDFRATPFIECNGGRGTC HYYANKYSFWLTTIPEQSFQ GSPSADTLKAGLIRTHISRC QVCMKNL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1486-1712 of human COL4A2 (NP_001837.2). |
Additional Info
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. Proteolytic processing produces the C-terminal NC1 peptide, canstatin. |
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. |
| Protein Name | Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-186797Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-3000157Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3000480Reactome: R-HSA-419037Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement Membrane |
| Alternative Antibody Names | Anti-Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin antibodyAnti-COL4A2 antibody |
Information sourced from Uniprot.org