| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-COL4A2 (1486-1712) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
COL4A2 |
| Gene ID: |
1284 |
| Uniprot ID: |
CO4A2_HUMAN |
| Immunogen Region: |
1486-1712 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1486-1712 of human COL4A2 (NP_001837.2). |
| Immunogen Sequence: |
VSIGYLLVKHSQTDQEPMCP VGMNKLWSGYSLLYFEGQEK AHNQDLGLAGSCLARFSTMP FLYCNPGDVCYYASRNDKSY WLSTTAPLPMMPVAEDEIKP YISRCSVCEAPAIAIAVHSQ DVSIPHCPAGWRSLWIGYSF LMHTAAGDEGGGQSLVSPGS CLEDFRATPFIECNGGRGTC HYYANKYSFWLTTIPEQSFQ GSPSADTLKAGLIRTHISRC QVCMKNL |
| Post Translational Modifications | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. Proteolytic processing produces the C-terminal NC1 peptide, canstatin. |
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. |
| Protein Name | Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474244Reactome: R-HSA-1650814Reactome: R-HSA-186797Reactome: R-HSA-2022090Reactome: R-HSA-216083Reactome: R-HSA-2214320Reactome: R-HSA-2243919Reactome: R-HSA-3000157Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-3000480Reactome: R-HSA-419037Reactome: R-HSA-8948216 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement Membrane |
| Alternative Antibody Names | Anti-Collagen Alpha-2(Iv Chain Cleaved Into - Canstatin antibodyAnti-COL4A2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
