• Western blot analysis of various lysates using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ119111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-COL1A2 antibody (1161-1260) (STJ119111)

SKU:
STJ119111

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL1A2 (1161-1260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL1A2
Gene ID: 1278
Uniprot ID: CO1A2_HUMAN
Immunogen Region: 1161-1260
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1161-1260 of human Collagen I/COL1A2 (NP_000080.2).
Immunogen Sequence: RTCRDLRLSHPEWSSGYYWI DPNQGCTMDAIKVYCDFSTG ETCIRAQPENIPAKNWYRSS KDKKHVWLGETINAGSQFEY NVEGVTSKEMATQLAFMRLL
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name Collagen Alpha-2(I Chain
Alpha-2 Type I Collagen
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-2173796
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-430116
Reactome: R-HSA-6785807
Reactome: R-HSA-75892
Reactome: R-HSA-76009
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-2(I Chain antibody
Anti-Alpha-2 Type I Collagen antibody
Anti-COL1A2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance