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Anti-COL1A1 antibody (STJ98915)

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-COL1A1 antibody (STJ98915)

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STJ98915

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	IF/IHC
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Collagen alpha-1I chain is suitable for use in Immunofluorescence and Immunohistochemistry research applications.

Clonality:	Monoclonal
Conjugation:	Unconjugated
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 500-2000 IHC-P 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	COL1A1
Gene ID:	1277
Uniprot ID:	CO1A1_HUMAN
Specificity:	The antibody detects endogenous Collagen I protein
Immunogen:	Synthetic Peptide of Collagen I

Post Translational Modifications	Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name	Collagen Alpha-1(I Chain Alpha-1 Type I Collagen
Database Links	Reactome: R-HSA-114604 Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-202733 Reactome: R-HSA-216083 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-3000480 Reactome: R-HSA-430116 Reactome: R-HSA-75892 Reactome: R-HSA-76009 Reactome: R-HSA-8874081 Reactome: R-HSA-8940973 Reactome: R-HSA-8948216
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Collagen Alpha-1(I Chain antibody Anti-Alpha-1 Type I Collagen antibody Anti-COL1A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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