• Immunofluorescence analysis of paraffin-embedded human colon using Human Pro-Collagen I alpha Rabbit polyclonal antibody (STJ11105505) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Human Pro-Collagen I alpha in paraffin-embedded human colon tissue using Human Pro-Collagen I alpha Rabbit polyclonal antibody (STJ11105505) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from U-138 MG cells using Human Pro-Collagen I alpha Rabbit polyclonal antibody (STJ11105505) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime:30s.

Anti-COL1A1 antibody (26-161) (STJ11105505)

SKU:
STJ11105505

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Collagen Alpha-1 (I Chain (26-161) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL1A1
Gene ID: 1277
Uniprot ID: CO1A1_HUMAN
Immunogen Region: 26-161
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-161 of human Collagen I/COL1A1 (NP_000079.2).
Immunogen Sequence: GQVEGQDEDIPPITCVQNGL RYHDRDVWKPEPCRICVCDN GKVLCDDVICDETKNCPGAE VPEGECCPVCPDGSESPTDQ ETTGVEGPKGDTGPRGPRGP AGPPGRDGIPGQPGLPGPPG PPGPPGPPGLGGNFAP
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Post Translational Modifications Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name Collagen Alpha-1(I Chain
Alpha-1 Type I Collagen
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-430116
Reactome: R-HSA-75892
Reactome: R-HSA-76009
Reactome: R-HSA-8874081
Reactome: R-HSA-8940973
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(I Chain antibody
Anti-Alpha-1 Type I Collagen antibody
Anti-COL1A1 antibody

Information sourced from Uniprot.org

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