• Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A1 Rabbit polyclonal antibody (STJ119220) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Collagen I/COL1A1 in paraffin-embedded human colon using Collagen I/COL1A1 Rabbit polyclonal antibody (STJ119220) at dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Collagen I/COL1A1 in paraffin-embedded human breast cancer using Collagen I/COL1A1 Rabbit polyclonal antibody (STJ119220) at dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Collagen I/COL1A1 Rabbit polyclonal antibody (STJ119220) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-COL1A1 antibody (1100-1200) (STJ119220)

SKU:
STJ119220

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL1A1 (1100-1200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:2000-1:10000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL1A1
Gene ID: 1277
Uniprot ID: CO1A1_HUMAN
Immunogen Region: 1100-1200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human Collagen I/COL1A1 (NP_000079.2).
Immunogen Sequence: GEQGDRGIKGHRGFSGLQGP PGPPGSPGEQGPSGASGPAG PRGPPGSAGAPGKDGLNGLP GPIGPPGPRGRTGDAGPVGP PGPPGPPGPPGPPSAGFDFS F
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Post Translational Modifications Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name Collagen Alpha-1(I Chain
Alpha-1 Type I Collagen
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-430116
Reactome: R-HSA-75892
Reactome: R-HSA-76009
Reactome: R-HSA-8874081
Reactome: R-HSA-8940973
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(I Chain antibody
Anti-Alpha-1 Type I Collagen antibody
Anti-COL1A1 antibody

Information sourced from Uniprot.org

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