• Western blot analysis of extracts of various cell lines, using COL11A2 antibody (STJ112500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COL11A2 antibody (210-380) (STJ112500)

SKU:
STJ112500

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL11A2 (210-380) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL11A2
Gene ID: 1302
Uniprot ID: COBA2_HUMAN
Immunogen Region: 210-380
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-380 of human COL11A2 (NP_542411.2).
Immunogen Sequence: IVPGVQAAYESCEQKELECE GGQRERPQNQQPHRAQRSPQ QQPSRLHRPQNQEPQSQPTE SLYYDYEPPYYDVMTTGTTP DYQDPTPGEEEEILESSLLP PLEEEQTDLQVPPTADRFQA EEYGEGGTDPPEGPYDYTYG YGDDYREETELGPALSAETA HSGAAAHGPRG
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. A disulfide-bonded peptide called proline/arginine-rich protein or PARP is released from the N-terminus during extracellular processing and is subsequently retained in the cartilage matrix from which it can be isolated in significant amounts.
Function May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Protein Name Collagen Alpha-2(Xi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-3000171
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-2(Xi Chain antibody
Anti-COL11A2 antibody

Information sourced from Uniprot.org

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