• Western blot analysis of HT-1080, using COL10A1 Rabbit polyclonal antibody (STJ11105243) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-COL10A1 antibody (1-100 aa) (STJ11105243)

SKU:
STJ11105243

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL10A1
Gene ID: 1300
Uniprot ID: COAA1_HUMAN
Immunogen Region: 1-100 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human COL10A1. (NP_000484.2).
Immunogen Sequence: MLPQIPFLLLVSLNLVHGVF YAERYQMPTGIKGPLPNTKT QFFIPYTIKSKGIAVRGEQG TPGPPGPAGPRGHPGPSGPP GKPGYGSPGLQGEPGLPGPP
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Protein Name Collagen Alpha-1(X Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000171
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(X Chain antibody
Anti-COL10A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance